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2x stop buffer  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Structured Review

    Thermo Fisher 2x stop buffer
    2x Stop Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2x+stop+buffer/Digitonin/bio_rxiv__64898__2026__02__07__704091-444-9-26
    Average 97 stars, based on 1 article reviews
    2x stop buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: The PRC2.1 Subcomplex Opposes G1 Progression through Regulation of CCND1 and CCND2
    Article Snippet: .. Samples were washed two more times and resuspended in 100 μL of Buffer 2 before starting the MNase digestion by adding CaCl 2 to a concentration of 2mM on ice for 30 minutes, after which the reaction was quenched with the addition of 100 μL 2X Stop Buffer (200mM NaCl, 20mM EDTA, 4mM EGTA, 50μg/mL RNase A, 40μg/mL GlycoBlue (Ambion), and 2pg/mL spike-in DNA) to inactivate the MNase. ..

    Article Title: PU.1 and BCL11B sequentially cooperate with RUNX1 to anchor mSWI/SNF to poise the T cell effector landscape
    Article Snippet: .. The reaction was stopped by the addition of 2x Stop Buffer (final concentration 100 mM NaCl, 10 mM EDTA, 2 mM EGTA, 20μg/mL glycogen, 25μg/mL RNase A (Thermo Fisher)) and the sample was incubated at 37°C for 20 min. Proteins in sample was then digested in 0.1% SDS and 250 μg/mL Proteinase K (New England Biolabs) for 2h at 56°C, shaking gently. ..

    Incubation:

    Article Title: A dual histone code specifies the binding of heterochromatin protein Rhino to a subset of piRNA source loci
    Article Snippet: .. Digestion was stopped by the addition of 2x STOP Buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% digitonin, 100 μg/ml RNase A (Thermo Fisher Scientific), 50 μg/ml glycogen) and samples were incubated at 37 °C for 30 min to release DNA fragments into the solution. ..

    Article Title: PU.1 and BCL11B sequentially cooperate with RUNX1 to anchor mSWI/SNF to poise the T cell effector landscape
    Article Snippet: .. The reaction was stopped by the addition of 2x Stop Buffer (final concentration 100 mM NaCl, 10 mM EDTA, 2 mM EGTA, 20μg/mL glycogen, 25μg/mL RNase A (Thermo Fisher)) and the sample was incubated at 37°C for 20 min. Proteins in sample was then digested in 0.1% SDS and 250 μg/mL Proteinase K (New England Biolabs) for 2h at 56°C, shaking gently. ..

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: .. To stop the enzymatic reaction, 100 μL of 2X STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% digitonin, 50 μg/mL RNase A (ThermoFisher Scientific #EN0531) and 50 μg/mL glycogen (Sigma #10901393001)) was added and samples were incubated at 37 °C for 30 minutes. ..

    Activity Assay:

    Article Title: A SWI/SNF-specific Ig-like domain, SWIFT, is a transcription factor binding platform
    Article Snippet: .. Equal volume of 2X Stop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.2% Triton-X 100, 100 μg/mL RNase A (Thermo Fisher Scientific), 50 μg/ml Glycogen (Thermo Fisher Scientific), 2 pg/mL yeast spike-in DNA (Cell Signaling)) was added to halt MNase activity. ..



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